Ravindra Ashok Sharma; Prajakta Kashinath Jagtap
Abstract
Recently, there has been an increase in research interest in metal nanoparticles and their synthesis because of their various applications in different industrial areas. The current study deals with the Actinomycetes-mediated synthesis of copper nanoparticles (CuNPs) isolated from mangrove soil and to ...
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Recently, there has been an increase in research interest in metal nanoparticles and their synthesis because of their various applications in different industrial areas. The current study deals with the Actinomycetes-mediated synthesis of copper nanoparticles (CuNPs) isolated from mangrove soil and to further access its application in different fields. Eight different soil samples were collected from three different mangrove sites located in Mumbai. A total of 15 different Actinomycetes isolates were obtained from soil samples and studied in the present investigation and were screened for metal tolerance. It was found that out of 15 isolates, only 3 were able to tolerate the highest metal salt concentration i.e. 10-1M. The synthesized CuNPs were further investigated with various characterizations such as UV-Vis spectroscopy, FTIR, and XRD. The identification of isolate GRC1 was done as per Bergey’s Manual of Systematic Bacteriology Volume 5 for preliminary identification of Actinomycetes and was identified as Streptomyces sp. This isolate was further characterized by Vitek MS and it was identified as Streptomyces verticillus. The inhibition zone by biosynthesized CuNPs was significantly greater when compared with standard antibiotics and CuSO4. The calculated degradation efficiency after 5hrs of incubation was 59.67% and 96.26% for Red M8B and Reactive green, respectively. Prevention of biofilm formation by CuNPs was confirmed by microscopic technique and significant inhibition of biofilm was observed. Thus, the mangrove Actinomycetes mediated bio-fabrication of CuNPs should gain much attention because of their unique properties like antimicrobial, anticancer, catalytic activity, wound healing, and antifouling.
Narasimha Raghavendra; Leena V Hublikar; S.M. Patil; Pritam Bhat
Abstract
Microwave assisted biosynthesis of nanoparticles has been a cost effective, environmentally benign, and alternative to the chemical method. In this context, we report eco-friendly and robust nanoparticles synthesized using the bio-waste (Banana leaves) extract material through a microwave method. The ...
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Microwave assisted biosynthesis of nanoparticles has been a cost effective, environmentally benign, and alternative to the chemical method. In this context, we report eco-friendly and robust nanoparticles synthesized using the bio-waste (Banana leaves) extract material through a microwave method. The newly synthesized Banana Leaves extract -Silver Nanoparticles (BL-AgNPs) is confirmed by using the UV-Visible, FT-IR spectroscopy and Scanning Electron Microscopy (SEM) techniques. UV-Vis spectrum shows the widening of the band around 476 nm, which confirms the polydispersed nature of BL-AgNPs. FT-IR spectroscopy explores that, hydroxyl and carbonyl groups in the Banana Leaves extract play vital role in the reduction of silver ions and also attach with AgNPs. The phytochemical studies reveal that, the polyphenols and alkaloids present in the BL extract act as reducing and stabilizing agent, which is responsible for the reduction of Ag+ (silver ions) to Ag (BL-AgNPs) and stabilization of BL-AgNPs. This clearly confirms the formation of silver nanoparticles (AgNPs). SEM results revealed that, bead shape of BL-AgNPs with particle size of 80 to 100 nm. In conclusion, BL-AgNPs exhibits promising anticancer activity against lung cancer and breast cancer cell line by endorsing inhibition of cell migration and proliferation on low concentration.