Smitha Vijayan; Divya Koilaparambil; Tijith K George; Jisha Manakulam Shaikmoideen
Abstract
The present study establish extracellular production of silver nanoparticles (AgNP) using Fusarium solani, from medicinal plant Withania somnifera (L.) (ashwagandha) and it’s antibacterial and cytotoxicity effects. Biological- AgNP (Bio- AgNP) were synthesized by using fungal cell free extract ...
Read More
The present study establish extracellular production of silver nanoparticles (AgNP) using Fusarium solani, from medicinal plant Withania somnifera (L.) (ashwagandha) and it’s antibacterial and cytotoxicity effects. Biological- AgNP (Bio- AgNP) were synthesized by using fungal cell free extract and characterized by SEM, TEM, UV spectroscopy, XRD, FTIR and AFM analysis. Antibacterial properties were assayed by well diffusion and cytotoxicity by RBC lysis test and MTT assay respectively. X- ray diffraction and microscopic analysis revealed the well dispersed and crystalline nature of spherical nanoparticles with a calculated size ranging from 10 - 50 nm. The Bio-AgNP exhibited significant antibacterial properties in a range of 50-100 µgml-1 against the selected clinical pathogens Escherichia coli,Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus and Klebsiella pneumoniae. The observed hemolysis of 3.906 % at 50 µg ml-1 suggested the safe therapeutic application of Bio - AgNP. MTT assay revealed that at the suggeseted concentration 69 % of cells are viable. These outcomes are extremely encouraging to utilize Bio-AgNP as a medication. Exploiting the endophytic organisms from therapeutic plants for improvement of nanomaterial is a uninvestigated and relatively novel territory. This may improve the likelihood in future to push the limit ahead in nanomedicine.
Koilparambil Divya; Liya C. Kurian; Smitha Vijayan; Jisha Manakulam Shaikmoideen
Abstract
The emerging infectious diseases and the development of drug resistance in the pathogenic microorganism is a matter of serious concern. Despite the increased knowledge of microbial pathogenesis and application of modern therapeutics, the morbidity and mortality associated with the microbial infections ...
Read More
The emerging infectious diseases and the development of drug resistance in the pathogenic microorganism is a matter of serious concern. Despite the increased knowledge of microbial pathogenesis and application of modern therapeutics, the morbidity and mortality associated with the microbial infections still remains high. Therefore, there is a pressing demand to discover novel strategies and identify new antimicrobial agents to develop the next generation of drugs or agents to control microbial infections. The use of nanoparticles is gaining impetus in the present century as they possess defined chemical, optical and mechanical properties. In the present study green synthesis of silver nanoparticles by Escherichia coli has been done. Various parameters such as mixing ratio of culture supernatant and silver nitrate, media, temperature and pH for production of silver nanoparticles were optimised. The nanoparticles synthesised was characterized using SEM, FTIR and XRD. The antibacterial activity of silver nanoparticles synthesised using both pellet and supernatant against human pathogens Salmonella typhi, Vibrio cholerae, Bacillus subtilis and Klebsiella pneumoniae was analysed and MIC was calculated as 20µg and 50µg respectively.