Recent interest in the photocatalytic water purification process can be related to its significant effectiveness over traditional wastewater techniques like activated carbon-based adsorption, chemical oxidation, microbial treatment, and others. In traditional methods have some difficulties such as incomplete removal, high consumption of chemical reagent, high treatment cost, time-consuming, and production of toxic secondary waste [1-3]. The phenomenal growth of the agrochemical industry is an outcome of widespread practice of intensive agriculture . As a consequence of which, the occurrence of pesticide residue as a pollutant in the water resources is becoming frequent. The photochemical degradation of toxic and hazardous compounds is a very useful and efficient technique to solve many environmental problems. The heterogeneous photocatalytic oxidation process mainly relies on the in-situ generation of hydroxyl radicals under ambient conditions which are capable of converting a wide spectrum of toxic organic compounds including the non-biodegradable ones into relatively innocuous end products such as CO2 and H2O [5,6]. In this context, photochemical degradation of pesticide residue is an extremely useful and efficient technique for the water treatment process [7-9]. Photocatalyst while activated by UV or visible radiation results in accelerated degradation of pesticide residue. Studies in wastewater treatment by TiO2 as photocatalyst are still some limitations such as low adsorption capacity to hydrophobic contaminants, absorption only a small fraction of sunlight (2–3%), high aggregation tendency and difficulty of separation and recovery[10-12]. To improve the photo efficiency of TiO2, many studies have been carried out to eliminate the limitations mentioned above.
TiO2 NPs are often implied as photocatalyst in the water treatment system employed exploiting advantages of nanoparticles in size, morphology, and other related properties [13,14]. These photocatalysts are basically semiconducting oxides and among these TiO2 occupied a pivotal position as n-type wide bandgap semiconductor for light absorption, surface adsorption, charge transport photo-induced super hydrophilicity, non-toxicity, etc. that resulted in excellent electrical and optical property.
TiO2 NPs successfully remove bacteria and different organic chemicals from water and air [16-19]. In most of these cases, the synthesis of TiO2 NPs for the stated purposes entails the involvement of organic solvents most of which toxic and hazardous and non-environment friendly [20,21]. Researchers have been going on for the substituent of toxic organic solvent with aqueous medium supported by benign stabilizing and capping agent like plant extract, green algae, micro-organisms such as fungi and bacteria [22-25], etc. Green synthesis of the TiO2 NPs has the advantage over other physicochemical processes because bio-inspired processes are relatively cheap, eco-friendly and renewable, compatible for pharmaceutical, water purification, and biomedical applications and can be easily scaled up for large scale synthesis. Most importantly such processes can be carried out at relatively low pressure and temperature .
In this present investigation synthesis of TiO2 NPs was carried out using Trema Orientalis(L) leaf extract which can be considered as a green method of the synthesis of NPs  and its effectiveness was studied by photodegradation of zoxamide [3, 5-dichloro-N-(3chloro-1-ethyl-1-methyl-2-oxopropyl)-4 methyl benzamide], a newly introduced benzamide chemical class of fungicide in India to control various fungal infection including blight in potatoes and tomatoes . Due to its chlorinated property, zoxamide can be a potentially hazardous water pollutant [29-30].
In the present work, the photocatalytic degradation of zoxamide in an organic aqueous medium containing under UV using TiO2 NPs synthesized from a green source of Trema Orientalis(L) plant was investigated. The photoproduct from zoxamide during this photocatalytic degradation was identified and the degradation of zoxamide pathway is proposed based on the identified photometabolite.
MATERIALS AND METHODS
Leaves of Trema Orientalis(L) were collected from West Bengal, India. Titanium (IV) isopropoxide and bulk TiO2 were purchased from Merk. Zoxamide of analytical grade is 98.6% pure from Sigma Aldrich. All solution and reaction mixture prepared using de-ionized water.
Synthesis of TiO2 NPs using aqueous extract of Trema Orientalis(L)
The fresh leaves of Trema Orientalis (L) were gently washed with deionized water to remove dust and then chopped. The chopped leaves (100g) and 500 mL deionized water were boiled at 60oC for 30 min and cooled. This extract was filtered through nylon mesh followed by Whatman 1 filter paper and stored at 4oC for further use. Titanium (IV) isopropoxide precursor is mixed with ethanol in the ratio 1:10 under stirring for 30 min. The aqueous plant extract was heated at 60oC for 10 min. Then plant extract and precursor solution were taken in the ratio 1:10 and stirred using a magnetic stirrer at 60oC for 4 h. Then the mixture was subjected to ultrasonication for 30 minutes. The TiO2 NPS was obtained by filtration and washed with ethanol. Separated TiO2 NPs were dried at 110oC and ground followed by calcination at 550oC for 3 h to remove the organic contaminants.
Characterization of synthesized NPs
The synthesized nanoparticles were characterized by X-ray diffraction [Rigaku Ultima III], Fourier Transform Infrared [IR prestige spectrophotometers-21], Scanning Electron microscopy [Carl Zeiss EVO-18], UV-VIS spectrophotometer[Perkin Elmer, Lambda 35] and Dynamic Light Scattering[Malvern particle size analyzer DTS,ver.5.00].
Photodegradation of pesticides under UV light
For the photo-degradation study of zoxamide in the different aqueous organic solvent, solvent systems having the composition of acetonitrile: water (1:1, V/V), methanol: water (1:1, V/V), isopropanol: water (1:1, V/V) was prepared. Solutions of zoxamide were made by dissolving 120 mg of Zoxamide in one liter of each aqueous organic solvent and every set of solution were used for the experiment. All sets of sample solutions of zoxamide were irradiated separately by UV light (λ max ≥ 250 nm), inside a reactor fitted with a high-pressure mercury lamp (125 Watt, HPK, Philips). This was jacketed with a water-cooled Pyrex filter. It helps to maintain a fixed solution temperature (25°C) with continuous stirring by a magnetic stirrer. Aluminum foil was used to cover the flasks tightly to prevent any type of contamination or other exposures. Samples were collected at intervals of 0, 1, 2, 3, 6, 9, 12, 15, 18, 20, 24, 27 h from the irradiated solution for determination of degradation pattern of zoxamide in different aqueous organic medium. The rate of dissipation of zoxamide in different aqueous organic solvent systems under UV irradiation shows that the half-life value of zoxamide in case of aqueous acetonitrile is 10.46 h, lowest compared to the two other solvents [for the aqueous methanolic half-life is 15.36h and for aqueous isopropanolic system half-life value is 17.20h]. Thus aqueous acetonitrile was taken as the solvent system for the rest of the experiment. Data regarding the photocatalytic degradation of zoxamide using bulk TiO2[50mg/L] in aqueous acetonitrile solution under UV irradiation at different pH[pH 9, pH 7, and pH 3] shows that the half-life value of zoxamide[120mg/L] at pH 9 is 3.25h, lowest compared to the two other[ half-life at pH 7 is 3.30h and at pH 3 is 3.36]( Supplementary Fig. 6).so the rest of the experiment done at pH 9. To understand the effect of bulk titanium dioxide as photocatalysts on photo-degradation of zoxamide, it was mixed (50mg/L) with one liter of aqueous acetonitrile solution which contains 120mg of zoxamide and follows a similar procedure for UV irradiation of the sample. Samples were collected at intervals of 0, 1, 2, 3, 6, 9, 12, 15h from the irradiated solution for determination of degradation pattern of zoxamide in the presence of bulk TiO2. A similar procedure was carried out separately for the rate determination and the kinetic study of photodegradation of zoxamide in aqueous acetonitrile solution using synthesized nano titanium dioxide(50mg/L) with the different initial concentration of zoxamide [sample no.Z1(110mg/L), Z2(105mg/L), Z3(165mg/L), and Z4(210mg/L)]. Samples were collected at intervals of 0, 1, 2, 3,4, 6, 8,10h from the irradiated solution for all the samples containing different concentrations of zoxamide.
To prepare the final sample, 1 ml of experimental solution was taken in a 50 ml centrifuge tube. Then, 10 ml of ethyl acetate was added to it. It was vortexed for 5 minutes. Next, the sample was centrifuged at 10000 rpm for 10 min. Then from centrifuged solution 5 ml supernatant solution taken and evaporate to dryness with the help of a turbo vapor nitrogen evaporator. Finally, volume was reconstituted with 5 ml acetonitrile. It was then filtered through a 0.2μ membrane filter. The acetonitrile fraction is the sample ready for analysis in the HPLC instrument for zoxamide residues.
JASCO HPLC combined with variable wavelength with Photo-diode array-Jasco MD-2010 plus-multi-wavelength detector (detector wavelength for zoxamide i.e λmax212 nm) and Chrompass software. The Pump used was of JascoPU – 2080 plus- intelligent HPLC pump. Solvent mixing was Module: Jasco MX-2080-31and Jasco AS-2050 Plus-intelligent sampler (Jasco LC –Net II/ADC). Peerless Basic C18 column with 250 mm × 4.6 mm × 5µm configuration was used. The mobile phase composition for zoxamide solution was acetonitrile/water (7:3 v/v). Flow Rate was 0.3 ml/min. Retention time observed for the zoxamide standard was 7.0 ± 0.1 min. Limit Of Detection (LOD) was 0.02 µg/ml and Limit Of Quantification was (LOQ) 0.05 µg/ml.
Waters Acquity Class Ultra Performance Liquid Chromatography (UPLC) coupled with Xevo TQD MS/MS using Mass Lynx™ V4.1 software. It (Electron impact, direct insertion) helped to characterize the structure of the photoproducts. In the Target lynx, spectral studies analysis, the molecular ion peak or fragment ion peak appeared at (M++1) and (M++23) due to the presence of hydrogen and sodium cation in the system.
Final analysis and calculation
As an external standard, a stock solution of 1 µg/ml standard analytical grade of zoxamide was used (98.6% purity). The retention time was recorded to be 7.0 ± 0.1 min. The injection volume of each cleaned up test sample was 20 µl for HPLC. The final zoxamide residues were identified by comparing the retention time of sample peaks with that of the standard peak. The number of residues in µg/ml (ppm) was calculated by following the equation given below.
A1 = Area of the sample in the Chromatogram
A2 = Area of standard in the Chromatogram
V1 = Total volume of sample in ml
C = Concentration of analytical standard of Zoxamide in µg/mL (µg/ ml)
W = Weight of the sample in g.
Rf = Recovery factor.
Calibration curve of the analytical standard of Zoxamide
To construct calibration curves, the zoxamide standard was diluted with acetonitrile. The linearity for test compounds was obtained by plotting the peak area against the concentration of the corresponding calibration standards. A good linearity curve was achieved in the range of 0.02 to 5.0 mg/L (Supplementary Table 1, SF1) with the correlation coefficient (R2) ≥ 0.998. The limit of detection was determined as 0.020 mg/L and 0.050 mg/L for Zoxamide.
RESULTS AND DISCUSSION
Powder X-ray Diffraction
The crystalline nature of the synthesized TiO2 NPs was analyzed using XRD. The XRD plot showed main peaks corresponding to 2θ value of 25.360, 36.200,37.200,54.240,56.610,69.530 and corresponding to (101),(112),(211), and (301) planes of TiO2 as shown in Fig.1a. The lattice parameters obtained for TiO2 NPs correspond to the anatase phase (JCPDS file no. 84-1285) [31-32]. The crystalline size of the synthesized TiO2 NPs was calculated using Scherrer’s equation and as well as Williaman-Hall(W-H) equation[33-34]. The crystalline size is found to be in 52nm as obtained using the Scherrer equation corresponding to the highest intensity peak at 2θ = 25.360. The average crystallite size of the synthesized TiO2 NPs estimated by the Williamson-Hall method (supplementary Fig.7) was about 59 nm. The calculated crystalline particle size obtained by Scherrer’s equation is smaller compare to the W-H equation as in Scherrer’s equation neglects the effect of crystallite size and lattice strain on the peak broadening. A few unassigned peaks were found in the vicinity of the characteristic peaks which suggest the presence of bio-organic phases occurs on the surface of the synthesized TiO2 NPs .
Fourier Transform Infrared Spectroscopy (FTIR) Analysis
Fig. 1 (b) represents the FTIR plot of the synthesized TiO2 NPs. The characteristic signal for TiO2 NPs due to the vibration of Ti-O-Ti bond observed at 450-700 cm-1.The band intensities of the spectrum for synthesized TiO2 NPs were 703.66 cm-1 δ (CH2) bending vibration, 831.72 cm-1 for C-H out of plane bending vibration , 1007.73 cm-1 for vs (C-O-C), 1059.35 cm-1 for va(C-O-C)  , the band at 1059.35 cm-1 C-N stretching vibration for primary amines , 1397.52 cm-1 for CH3 umbrella deformation, 1633.97 cm-1 for deformation vibration of the Ti-OH stretching mode , 1650.84 cm-1for stretching vibration of C=O and C-N , 2748.36 cm-1 for alkyl C-H stretching and 3225.29 cm-1 for O-H alcohol . A comparison of these results with earlier reports indicated that alcohols, carboxylic acids, phenols, amines played an important role in the green synthesis of TiO2 NPs as capping and stabilizing agents  The TremaOrientalis contains (-)-ampelopsin F, (-)-epicatechin, (+)-catechin, (+)-syringaresinol, N-(trans-p-coumaroyl)tyramine, N-(trans-p-coumaroyl) octopamine, trans-4-hydroxy-cinnamic acid and 3,5-dimethoxy-4-hydroxyphenyl-1-o-β-D-glucoside. Among these catechins, N-(trans-p-coumaroyl)tyramine, trans-4-hydroxy-cinnamic acid, and 3,5-dimethoxy-4-hydroxyphenyl-1-o-β-D-glucoside are water-soluble. The FTIR spectrum of green synthesized TiO2 clearly implying the presence of flavonoid/terpenoids on the nanoparticle surface and above water-soluble organic compound present in leave extract have a crucial role in reducing as well as a stabilizing agent to synthesized TiO2 NPs.
Scanning Electron Microscope (SEM)
The surface morphology of synthesized TiO2 was investigated using the SEM image (Fig.2). The obtained TiO2 nanoparticles were observed with irregular particle structure. The nanoparticles were dispersed evenly on the surface with the development of aggregate nanoparticles which revealed that powder particles are marginally agglomerated.
The absorption spectrum of TiO2 NPs synthesized using Trema Orientalis leaf extract is analyzed using UV-Vis spectroscopy, which is shown in Fig.3a. The absorption band was estimated near 351nm. To calculate the optical bandgap from the UV-vis spectra of synthesized TiO2 NPs, we used Tauc relation , which is given by the following Kubelka-Munk equation [45 ]. In the Tauc equations (Equation 1), α is the absorption coefficient, A is the absorbance, d is the thickness of the sample, through which the UV light is passing, and hν (eV) is photon energy.
α = - ln(1 - A)/d (1)
To calculate the bandgap, we need to fit the data in Tauc Plot [(αhc/λ)2 vs hc/λ (for direct bandgap) or (αhc/λ)1/2 vs hc/λ (for indirect bandgap)]. The absolute magnitude of d might not be important here, as the bandgap is dependent only on the x-intercept of Tauc Plot. So, assume d = 1 in that given equation, and the data can be plotted to calculate the bandgap. The calculated direct bandgap value for synthesized TiO2 NPs is 3.27 eV (Fig.3b) which falls in the UV light region of the electromagnetic spectrum and this made the photo-catalyst TiO2 highly active under UV light.
Histogram of synthesized TiO2 NPs representing particle size distribution of the sample is shown in Fig.3c. It is observed from the histogram study that the maximum volume fraction of particles existed in the size range of 88 to 94 nm range. Immediate after
the synthesis process the particles relatively wide size range but with time the size distribution became narrow following subsequent growth of the synthesized nanoparticles.
Column chromatographic isolation and characterization of the desired photometabolite
It was found from the kinetic experiment that the half-life value of zoxamide was lowest under UV irradiation in acetonitrile-water containing synthesized TiO2 as photocatalyst (Table 1). So, it was expected that the maximum quantitative yield of photo metabolite will be under UV irradiation in the presence of synthesized TiO2 which was also supported by Micromass spectroscopic analysis. For the isolation of photometabolite, this system was chosen for the column chromatographic study. For extraction, after 24 h of UV irradiation, the system was taken. The extracted fraction was concentrated and the crude concentrate was subjected to column chromatography over silica gel. The column was eluted with different solvent systems with increasing polarity (given in supplementary Table ST2). On concentration of the fraction 91-110 with hexane: ethyl acetate (7:3) obtained from the above column chromatography yielded white solid needle shape crystals (M1).
Examination of photolytic product from fraction 91-110
The melting point of metabolite (M1): 1350C
Identification of photo product
The photoproduct (M1) from the fraction of hexane: ethyl acetate (7:3) was a crystal. For identification of the crystal M1, it was subjected to an X-ray diffraction (XRD) study . Crystal data were collected on a Bruker SMART APEXIICCD area-detector diffractometer using graphite monochromated Mo Kα radiation (λ= ٠.٧١٠٧٣ Å). X-ray data reduction was carried out using the Bruker SAINT program. The structures were solved by direct methods using the SHELXS-97 program, and refinement using SHELXL-97 program. Selected crystal data and data collection parameters for the compound M1 (given in Supplementary Table ST3).X-ray data reduction, structure solution, and refinement were done using the SHELXL-97 program package. The three-dimensional crystal structure of the photo product M1 is shown in Fig.5a and 5b.
The X-ray crystallographic data conclusively identifies the photo product M1 as 3,5-dichloro-4 methyl benzoic acid.
Aqueous suspension of TiO2 NPs generates electron-hole pair  during irradiation at a wavelength less than the bandgap energy (λ <410 nm).
TiO2+h ν −→ TiO2(e-cb + h+νb) −→ e-cb+ h+νb
After the reaction of the valence band holes (h+νb) and the TiO2 surface-active OH- groups (or oxidize able species, viz., H2O, H2O2, or organic substrates) recombine to form.OH radicals.
h+νb + OH-(surface) (or H2O) −→ .OH + H+
The electrons may also react with reducing species (such as O2 or H+) in the solution yielding OH− or H radicals as follows:
O2 + H2O + 2e-cb −→ 2OH− and H+ + e- −→ H•
The reaction proceeds via homolytic cleavage of the C-N bond for zoxamide and formation of stable aromatic substituted benzoyl radical substrate here. The other part is not detected here. The hydroxyl radical is being a very powerful oxidant, oxidize most of the organic molecules to give the oxidative products as follows:
•OH + Substrate −→ Oxidative products.
The product here is 3,5-dichloro 4 methyl benzoic acid.
Plausible mechanistic pathway:
Kinetic study of photocatalytic degradation of Zoxamide
To obtain information regarding the kinetics of photocatalytic degradation of zoxamide, experimental results were fitted with zero, first and second-order equation. Photocatalytic degradation of zoxamide was fitted well with the pseudo-first-order reaction kinetics: lnC0/C = Kobst [where C0is the initial concentration of zoxamide (mg/L) and C1,C2, C3, and C4 are the concentration of zoxamide after irradiation at the time, t for Z1,Z2,Z3, and Z4 respectively] in Fig. 6 and reaction rate and R2 data in Table 2. The pseudo-first-order rate constant Kobs is determined by linear fitting the experimental data with this model. The
good regression coefficient (R2 = 0.989) indicated that the photocatalytic degradation of zoxamide by the TiO2 NPs fitted into the pseudo-first-order rate[48-49] and the rate constant value (Kobs) for the first-order reaction is higher to compare with both zero-order and second-order rate constant.
It is noteworthy that zoxamide with the lowest concentration (105mg/L) exhibits the highest reaction rate (0.32048 h-1), which is greater than that of 210 mg/L zoxamide. The reaction rate is decreased at higher concentrations. This indicated that the decrease in the number of active sites on the photocatalyst surface with the increasing initial concentration of zoxamide in the batch process photo-degradation experiments. Similar results have been reported by other researchers for photocatalytic degradation [50-52]. The influence of the initial concentration of zoxamide on the photocatalytic degradation rate is described by the pseudo-first-order kinetic model for heterogeneous photocatalysis involving Langmuir-Hinshelwood [L-H] kinetic expression has been used successfully by many authors [53-54]. The L-H model for photodegradation of zoxamide can be written as below:
Where, [Zoxamide]0 is the initial concentration of Zoxamide,
Kz , the L-H adsorption equilibrium constant.
K, the rate constant of surface reaction and
Kobs , the pseudo-first-order rate constant.
The applicability of the above L-H equation for the photodegradation of zoxamide has been confirmed by the linear plot obtained by plotting vs initial concentration of zoxamide (Fig.7). The rate constant (K) is 20.21µg/ml/h and the equilibrium constant (Kz) is 0.01863 ml/µg obtained from the slope and intercept of the linear fit straight line. The value of the correlation coefficient (R2) is 0.998 suggested that the photocatalytic degradation of zoxamide fits the L-H kinetic expression well.
The investigation of photocatalytic activity of green synthesized TiO2 NPs on zoxamide had developed the following findings:
1. TiO NPs were synthesized successfully by the green method using Titanium (IV) isopropoxide and Trema Orientalis(L) leaf extract and confirmed by XRD, FTIR, and UV-Vis plot.
2. The particle size of synthesized TiO2 NPs was calculated from the XRD plot by both the Scherrer equation (52nm) and the W-H equation (59 nm).DLS study reveals that the particle size ranges from 88-94 nm.
3. FTIR data confirm the presence of Ti-O bonds in the powder sample.
4. SEM image showed that the synthesized TiO2 NPs were of irregular shape.
5. The bandgap of the synthesized TiO2 NPs was measured using the Tauc plot and it comes out to be 3.27 eV.
6. HPLC and Mass spectra data were used to identify the photo metabolite (3,5-dichloro-4 methyl benzoic acid) and a plausible pathway for the photodegradation was also discussed.
7. The kinetic study of photodegradation of zoxamide using synthesized TiO2 NPs obeys Langmuir–Hinshelwood model and fitted the pseudo-first-order rate constants
We are grateful to infrastructure facilities provided by the School of Material Science and Nanotechnology, Jadavpur University and Export Testing Laboratory (NABL accredited ISO 17025 certified Laboratory), Department of Agricultural Chemicals, Bidhan Chandra Krishi Viswavidyalaya, West Bengal, India and Soumik Mandal ( IISER Kolkata) for XRD analysis.
There is no potential conflict of interest reported by any author.