TY - JOUR ID - 30950 TI - Genomic Effect of Silver Nanoparticles in Staphylococcus aureus Bacteria JO - Journal of Water and Environmental Nanotechnology JA - JWENT LA - en SN - 2476-7204 AU - Alizadeh, Vida AU - Golestani Eimani, Bahram AU - Amjady, Fariba AD - Department of Biology, Urmia Branch, Islamic Azad University, Urmia, Iran Y1 - 2018 PY - 2018 VL - 3 IS - 1 SP - 51 EP - 57 KW - Genetic diversity KW - Growth Inhibition KW - NTSYS-PC Software KW - Random Amplified Polymorphic DNA (RAPD-PCR) KW - Silver nanoparticles DO - 10.22090/jwent.2018.01.005 N2 - Background and objectives: Drug resistance in bacteria is one of the important problems in the antibacterial field. Therefore, new drugs and therapeutic approaches are required to eliminate bacteria using different and novel mechanisms. Among these, the silver nanoparticles have been proposed as a substance with antibacterial properties against gram-positive and gram-negative bacteria. The present study aimed to investigate the effects of silver nanoparticles with a size of less than 20 nm on the genome of Staphylococcus aureus (S. aureus) as a model for gram-positive bacteria.Material and methods: For this purpose, the bacteria were treated at concentrations of 100 and 150 µg/ml nanoparticles and antimicrobial properties of the nanoparticles were investigated in intervals of 2, 4 and 24 hours, then DNA was extracted. RAPD molecular marker was used to investigate the effects of nanoparticles on the genome. In addition, the results of electrophoresis for polymerase chain reaction (PCR) products on agarose gel were analyzed.Results: The present findings demonstrated that silver nanoparticles not only have an inhibitory effect on bacteria but also affect the genomic DNA sequence of this bacterium and change it in different sites.Conclusion: The nanoparticles are antibacterial compounds and can be an appropriate alternative to antibiotics. UR - https://www.jwent.net/article_30950.html L1 - https://www.jwent.net/article_30950_cc164ffb0807f4c58f8980eb5d211376.pdf ER -