P. Koteswara Rao; B Vikram Babu; A. Rama Krishna; M. Sushma Reddi; B. Sathish Mohan; K. Anjani Devi; U. Susmitha; T. Raghava Rao
Abstract
The present study explores the green approach for the preparation of silver nanoparticles (AgNPs) through the reduction of silver nitrate by the cell-free stem and leaf aqueous extracts of Litsea glutinosa (L.glutinosa) and its potential antibacterial activity. The analytical instruments include scanning ...
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The present study explores the green approach for the preparation of silver nanoparticles (AgNPs) through the reduction of silver nitrate by the cell-free stem and leaf aqueous extracts of Litsea glutinosa (L.glutinosa) and its potential antibacterial activity. The analytical instruments include scanning electron microscopy, Fourier transforms infrared spectroscopy, UV-visible spectroscopy, and X-ray diffraction spectroscopy confirmed the synthesis of smaller, uniformly spherical AgNPs (10-40 nm). The average crystalline size of prepared AgNPs produced by L. glutinosa leaf extract was found to be 19 mm. From UV-visible spectral analysis, the maximum absorbance peak appeared at 444 nm for leaf extract AgNPs different from stem extract AgNPs (422 nm), which are found to be specific for AgNPs. The L.glutinosa stem extract-assisted AgNPs have shown significant antibacterial activity against Bacillus subtilis (Gram-positive) and Escherichia coli (Gram-negative) in comparison to Gentamycin. Hence, the AgNPs obtained by green synthesis can be therapeutically explored against bacterial infections.
Suresh Ghotekar; Ajay Savale; Shreyas Pansambal
Abstract
The aim of this study was to expand an ecofriendly route for the fabrication of spherical shape silver nanoparticles (AgNPs) using an aqueous extract of Leucaena leucocephala L. leaves to act as stabilizing and reducing agent. Several biomolecules present in plant extract are accountable for single step ...
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The aim of this study was to expand an ecofriendly route for the fabrication of spherical shape silver nanoparticles (AgNPs) using an aqueous extract of Leucaena leucocephala L. leaves to act as stabilizing and reducing agent. Several biomolecules present in plant extract are accountable for single step reduction of metal ions into nanoparticles. The synthesized AgNPs were characterized by X-ray diffraction (XRD) profile, Fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), Energy-dispersive X-ray spectroscopy (EDS) and Photoluminescence. Besides these, AgNPs evinced potent antibacterial, antimalarial and antimycobacterial activity against Pseudomonas aeruginosa, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Salmonella typhi, Bacillus subtilis, Plasmodium falciparum and Mycobacterium tuberculosis. The results suggest that the efficiently synthesized AgNPs can be used as potential candidates for various medicinal applications in bionanotechnology based industries.