Ravindra Ashok Sharma; Prajakta Kashinath Jagtap
Abstract
Recently, there has been an increase in research interest in metal nanoparticles and their synthesis because of their various applications in different industrial areas. The current study deals with the Actinomycetes-mediated synthesis of copper nanoparticles (CuNPs) isolated from mangrove soil and to ...
Read More
Recently, there has been an increase in research interest in metal nanoparticles and their synthesis because of their various applications in different industrial areas. The current study deals with the Actinomycetes-mediated synthesis of copper nanoparticles (CuNPs) isolated from mangrove soil and to further access its application in different fields. Eight different soil samples were collected from three different mangrove sites located in Mumbai. A total of 15 different Actinomycetes isolates were obtained from soil samples and studied in the present investigation and were screened for metal tolerance. It was found that out of 15 isolates, only 3 were able to tolerate the highest metal salt concentration i.e. 10-1M. The synthesized CuNPs were further investigated with various characterizations such as UV-Vis spectroscopy, FTIR, and XRD. The identification of isolate GRC1 was done as per Bergey’s Manual of Systematic Bacteriology Volume 5 for preliminary identification of Actinomycetes and was identified as Streptomyces sp. This isolate was further characterized by Vitek MS and it was identified as Streptomyces verticillus. The inhibition zone by biosynthesized CuNPs was significantly greater when compared with standard antibiotics and CuSO4. The calculated degradation efficiency after 5hrs of incubation was 59.67% and 96.26% for Red M8B and Reactive green, respectively. Prevention of biofilm formation by CuNPs was confirmed by microscopic technique and significant inhibition of biofilm was observed. Thus, the mangrove Actinomycetes mediated bio-fabrication of CuNPs should gain much attention because of their unique properties like antimicrobial, anticancer, catalytic activity, wound healing, and antifouling.
Sathiya Sheela D; P Viswanathan; K Kalimuthu; A Vanitha
Abstract
The development of biologically enthused green synthesis of silver nanoparticles (SNPs) has concerned significant global awareness about medical science and disease treatment. This paper discusses the green synthesis of SNPs using organic green sources; here we report a facile bottom-up ‘green’ ...
Read More
The development of biologically enthused green synthesis of silver nanoparticles (SNPs) has concerned significant global awareness about medical science and disease treatment. This paper discusses the green synthesis of SNPs using organic green sources; here we report a facile bottom-up ‘green’ route for the synthesis of SNPs using aqueous leaves extract of Pogostemon speciosus (Benth.) and evaluate its in-vitro anti-inflammatory, antibacterial and photocatalytic activities. The nanoparticles were investigated for the preparation of denaturation particles with PSLASNPs and the evaluation of anti-inflammatory activity with Protein denaturation and HRBC stabilization assays. Later, these PSLASNPs were studied for their potential role in antibacterial activity by well diffusion method, and Photocatalytic activity on degradation of dyes was demonstrated by using dyes Crystal violet, Coomassie blue, and Congo red. At 1000 µg/ml, the PSLASNPs have the greatest prevention of protein denaturation (71.92±1.37%), whilst the stabilization of the HRBC membrane exhibited significant anti-inflammatory action (64.39±1.61 %). The PSLASNPs showed the best antibacterial activity at the concentration of 10 µg/ml against Bacillus subtilis (8.2 mm), followed by Pseudomonas stuberia (6.2 mm) and Escherichia coli (6.4 mm), Staphylococcus aureus (5.3 mm), Staphylococcus gallinarium (4.5 mm) respectively at the same concentrations. Crystal violet, Coomassie blue, and Congo red were used for Photocatalytic activity on the breakdown of dyes. After 35 minutes, the degradation process was determined to be complete by the transformation of the reaction mixture's color to colorless. As a result, the PSLASNPs have anti-inflammatory, antibacterial, and photocatalytic activities.
Latha Vatchalan; Pandiselvam S.
Abstract
Paper industries will be using a different kind of dyes for producing various kinds of paper. Rhodamine–B (Rh-B) dye is one of the major sources of color effluents from textile and paper dyeing industries, and they cause long-term effects for on an aquatic environment. So this work mainly focused ...
Read More
Paper industries will be using a different kind of dyes for producing various kinds of paper. Rhodamine–B (Rh-B) dye is one of the major sources of color effluents from textile and paper dyeing industries, and they cause long-term effects for on an aquatic environment. So this work mainly focused on the synthesis of carbon nanoparticles from neem leaves, characterization and its adsorption and photocatalytic action against Rh-B dye. In this study, Carbon nanoparticles (C NPs) from neem leaves were synthesized for adsorption and photo catalytic degradation of Rh-B dye used in paper industries. The synthesized carbon NPs were characterized by the powder X-Ray Diffraction and Scanning Electron Microscopy. The adsorption and photocatalytic properties of Ccarbon NPs were examined for 1 hour by studying the degradation of the Rh-B dye at every 5 minutes time interval through UV-Visible spectrophotometer. The results obtained shown that carbonC NPs acts as better adsorbents than as photo catalysts.